Ion-exchange Chromatography of Sugars, Sugar-Phosphates and Nucleotides
نویسندگان
چکیده
منابع مشابه
Ion Exchange Chromatography
The separation and purification of various elements by ion exchange chromatography takes advantage of the variation of the electrostatic bond energies of ions in solution. Thus, the two main controlling factors in ion exchange chromatography are the ionic charge (z) and the ionic radius (r). These two are combined in the ionic potential – z/r – that can be used to compare relative electrostatic...
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Chromatography is the separation of a mixture of compounds into its individual components based on their relative interactions with an inert matrix. However, chromatography is more than a simple technique, it is an important part of science encompassing chemistry, physical chemistry, chemical engineering, biochemistry and cutting through different fields. It is worth to be mentioned here that t...
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The separation of myo-inositol phosphates by ion pair, reverse-phase high performance liquid chromatography has been investigated. The retention of the inositol phosphates is dependent on both the polarity of the hetaeron utilized and on the pH of the solvent. A method is presented which permits the isocratic separation of multiple forms of inositol phosphates including isomers of myo-inositol ...
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The aim of this work is to establish a quantitative method to determine the ratio of [U-(13)C] labeled to unlabeled hexose monophosphates isolated from yeast extracts. This is accomplished by anion exchange chromatography and mobile phase desalting followed by electrospray (ESI) mass spectrometry. We test the method with the analysis of a sample of biological origin. Previously developed analyt...
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Mixtures of aldonic and aldaric acids are difficult to analyse. Aldonic acids can be separated in a satisfactory way on anion-exchange resins with a solution of sodium chloride as eluent. Under these circumstances, however, aldaric acids show unacceptably long elution times and peak broadening. The ability of aldaric acids to form non-adsorbable complexes with magnesium ions lowers their elutio...
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ژورنال
عنوان ژورنال: Journal of the agricultural chemical society of Japan
سال: 1960
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.34.4_a5